CNCA

CNCA aligns multiple small, closely related annotated genomes (<50kb, typically virus) using information from protein alignments.
To execute a simple run of CNCA aligner, submit at least two GenBank files and click on RUN.
For more details, see the documentation.

Select File
Default parameters.
Scoring matrix
Parameters:

Scoring matrix for amino acid sequences:

Scoring matrix for nucleotide sequences:

Switch it to '1PAM / κ=2' when aligning closely related DNA sequences.

Gap opening penalty: (1.0 – 5.0)

Offset value: (0.0 – 1.0)

Alignment strategy


Progressive methods


Iterative refinement methods